Viral integration may also lead to modified expression of cellular genes nearby, disruption of genes, as well as genomic amplifications that may promote oncogenesis 23, 27. Upon integration, disruption or complete deletion of the E1 or E2 gene is often observed, resulting in constitutive expression of the E6 and E7 oncogenes 24– 26, inactivation of cell cycle checkpoints and genetic instability 23. In contrast to a limited number of studies on HPV variability, HPV integration into the host genome has been more widely studied and is regarded as a determining event in cervical carcinogenesis 21– 23. However, recent studies have revealed variability below the level of variant lineages that may be evidence of intra-host viral evolution and adaptation 17– 20. Traditionally, studies have focused on cancer risk of main variants. Isolates of the same HPV types that differ by 1–10% or 0.5–1% across the genome are referred to as variant lineages or sublineages, respectively 11, 12.ĭespite phylogenetic relatedness, HPV variant lineages can differ in their carcinogenic potential 13– 16. ![]() Each individual HPV type shares at least 90% sequence identity in the conserved L1 open reading frame (ORF) nucleotide sequence. To date, more than 200 HPV types have been identified 10. HPV contains an approximately 7.9 kb circular double-stranded DNA genome, consisting of early region (E1, E2, E4-7) genes, late region (L1, L2) genes and an upstream regulatory region (URR) 9. An appropriate sequencing approach is needed to uncover these genomic events during a persistent HPV infection. This indicates that in addition to HPV infection, additional factors such as HPV genomic variability and integration, could contribute to the HPV-induced carcinogenic process. ![]() However, only a small fraction of HPV infections at any site will progress to cancer 8. HPV infection is also associated with cancer in penis, vulva, vagina, anus, and head and neck 7. Of the 13 carcinogenic high-risk types, HPV16 and 18 are associated with about 70% of all cervical cancers 5, 6. A persistent infection with HPV high-risk genotypes is recognised as a necessary cause of cancer development 4. Human papillomavirus (HPV) is the main cause of cervical cancer 1, one of the most common cancers in women worldwide, causing more than 200,000 deaths each year 2, 3. Efficient identification of both HPV variability and integration sites will be important for the study of HPV evolution and adaptability and may be an important tool for use in cervical cancer diagnostics. Comprehensive studies on HPV intra-host variability generated during a persistent infection will improve our understanding of viral carcinogenesis. In contrast to other approaches, TaME-seq proved to be highly efficient in HPV target enrichment, leading to reduced sequencing costs. HPV genomic variability was observed in all samples allowing identification of low frequency variants. ![]() Chromosomal integration breakpoints and large deletions were identified in HPV positive cell lines and in one clinical sample. Our results showed deep HPV genome-wide sequencing coverage. For method validation, cell lines (n = 4), plasmids (n = 3), and HPV16, 18, 31, 33 and 45 positive clinical samples (n = 21) were analysed. TaME-seq combines tagmentation and multiplex PCR enrichment for simultaneous analysis of HPV variation and chromosomal integration, and it can also be adapted to other viruses. To uncover these genomic events in an HPV infection, we have developed an innovative and cost-effective sequencing approach named TaME-seq (tagmentation-assisted multiplex PCR enrichment sequencing). ![]() HPV genomic variability and chromosomal integration are important in the HPV-induced carcinogenic process.
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It’s essential to do your research, ask for recommendations, and visit potential breeders to ensure they are ethical and responsible.Įmbarking on the journey to bring a Tabby Maine Coon kitten into your home is exciting. Note: This list is illustrative and not exhaustive. While Tabby Maine Coons may have a higher price due to their popularity, remember that the most important aspect is the health and personality of your future feline companion. Prices can vary greatly based on factors like pedigree, color, and pattern. Table 1: Breeders Specializing in Tabby Maine Coons Breeder Name It’s a beautiful reminder of the intricate natural processes that create the stunning feline companions we know and love. While genetics in cats can be complex, this basic understanding gives you an insight into how each Tabby Maine Coon gets its unique coat color and pattern. Ticked pattern with color bands on each hair, minimal stripes Mackerel pattern with thin, parallel stripesĬlassic or blotched pattern with thick, swirling stripes Table 2: Gene Influences on Tabby Patterns Pattern The specific versions of these genes a cat inherits from its parents will determine the type of tabby pattern it will display. The specific type of tabby pattern (mackerel, classic, or ticked) is controlled by the Tabby (Ta) gene. The ‘non-agouti’ or ‘hypermelanistic’ variant (a), if present, would lead to a solid color coat, suppressing the tabby pattern. This gene influences the distribution of color on each individual hair. The tabby pattern in cats, including Maine Coons, is controlled by the Agouti (A) gene. ![]() Regardless, it’s just one of the many endearing characteristics that make Maine Coons – ‘M’ mark or not – utterly irresistible to cat lovers worldwide. In essence, while the ‘M’ mark is a common and delightful feature of Tabby Maine Coons, it’s not a universal trait across the entire breed. Plus, variations in fur length, density, and color blending can sometimes make the ‘M’ less distinct. Solid colored Maine Coons and those with certain other patterns might not exhibit a visible ‘M’ mark. However, not every Maine Coon will have a noticeable ‘M’. The ‘M’ is especially prominent in brown, blue, red, and cream tabbies, and a bit subtler in silver tabbies. The ‘M’ mark is associated with tabby patterns, and since Maine Coons predominantly exhibit tabby patterns, you’ll find that most Maine Coon cats do indeed sport this charming mark. But does every Maine Coon have this marking? Let’s unravel this feline mystery. This is a traditional coat color and pattern and it is believed most early Maine Coons were of this coat type. Cats with an all-ticked pattern almost shimmer in the sunlight because of the color variation.Do All Maine Coons Have An M On Their Forehead?Ī fascinating characteristic common among tabbies, including our beloved Maine Coon Tabbies, is the signature ‘M’ on their forehead. The black tabby Maine Coon is sometimes referred to as a brown tabby. If you look closely, you'll see different bands of color down the length of the cat's hairs.
![]() Montage songs can be slow and sentimental, or upbeat and lively, depending on the photos you choose. Some popular montage themes we help our clients create include traditional ones like Infinite Collage, Light Panes, and Keepsake, or specific ones like Life’s A Beach, A Wonderland of Snow, and Bon Voyage. Some families select a visual theme for their mitzvah montage, which can make it a little more interesting and engaging to watch than a series of photos scrolling by. 001.jpg through 100.jpg) before you share them with your entertainment team. To organize the pictures chronologically, you’ll want to change the file name of each picture to reflect the corresponding order (i.e. A random order can be a fun surprise on the day of the mitzvah, but choosing a specific order allows you to tell a special story about your child’s life. The photos you choose for the montage can be displayed either chronologically or random. Include as many pictures of your family and friends as possible - everyone loves seeing themselves represented on screen! ![]() This number of photos typically produces a montage that is approximately seven minutes long and is accompanied by two songs. We recommend selecting a total of 100 horizontally-oriented, high-resolution pictures for your montage. Here are some of the elements you’ll need to consider when preparing your montage materials: If you’re hiring an entertainment team for your child’s mitzvah, they will be able to help you create, edit, and play the photo montage during the event. The soundtrack to The Land Before Time, this song serves as a beautiful reminder about listening to your heart, recognizing your dreams, which deserve to be fulfilled, and truly living your life with a purpose. Now you've got some Quinceañera party songs, it's time to find a DJ, bands and entertainers for your event on The Bash.To help, here are our best tips for planning your photo montage, along with our recommendations for the best mitzvah montage songs to use. Searching for whats right in front of your face. Listen to our Quinceañera playlist on Spotify here. Better When I’m Dancin’ – Meghan Trainor. ![]() On The Floor (Radio Edit) – Jennifer Lopez, Pitbull.Pocketful of Sunshine – Natasha Bedingfield.Taki Taki (with Selena Gomez, Ozuna & Cardi B) – DJ Snake.In My Daughter's Eyes – Martina McBride. ![]() This playlist has some classic tunes that are sure to please everyone from Mom and Dad to the birthday girl herself! But, don’t forget to also mix in some of your favorite current pop tunes that will get your guests moving on the dance floor! Here are some of the best songs to add to your Quinceañera playlist: We’ve put together some song suggestions for your Quinceanera playlist with a mix of English and Spanish songs. When you hire a band or DJ you want to make sure that they have this perfect Quinceañera playlist for your event. For many, this coming of age celebration has become quite the social event – some of these parties are almost as elaborate as weddings, and so bands, DJs and variety entertainers are all very popular selections for a Quinceañera. One of the most important parts of planning a fabulous Quince party is hiring the right entertainment and cueing up the best Quinceañera playlist. Quinceañera (15th birthday) is a wonderful milestone event for a girl in the Latin community. ![]() ![]() VIDEO 15 : amp test thursday soundstream reference 300. VIDEO 14 : soundstream reference 500sx test 9-22-16. tested today is the orion 275sx (gen 2) from the early 1990's. VIDEO 13 : orion 275sx rms power output bench test - old school 75x2 car amplifier - see the 4th of my 75x2 watt rms amplifiers from the 1990's. VIDEO 12 : back from the dead! classic soundstream reference class a 5.0 repaired - my buddy juan did it again! he repaired 3 amps for me that had no hope of ever working again. VIDEO 11 : amp test tuesday - soundstream class a 5.0 - rated 500 watts - 1/4 ohm - smd ad-1 amp dyno - see my test cart built from scratch here. ![]() VIDEO 10 : power x -site protect / langkah perbaikan - link share : contoh video servis. VIDEO 09 : 1996 soundstream reference class a 10.0 amp dyno test - possibly the ultimate 50 watt "cheater" amp? let's try out the 1996possibly the ultimate 50 watt "cheater" amp? let's try out the 1996soundstream referenceclass a 10.0. this will be a series in about 1 year yep thanks for watching!. VIDEO 08 : soundstream reference collection - i have two references 700sx and one 644s. VIDEO 07 : soundstream reference 300 amp test - update - i decided to re-test thei decided to re-test thesoundstream reference300 once i noticed the switch wasn't set to "mono". VIDEO 06 : 405 x-site et kit soundstream. VIDEO 05 : soundstream reference 300 power output test - old school - see another of my old school 75x2 amps, thesee another of my old school 75x2 amps, thesoundstream reference300 on the bench. VIDEO 04 : soundstream amp collection - soundstreamclass a 10.0, picasso,soundstreamclass a 10.0, picasso,405, 160s, amp collection. VIDEO 03 : soundstream reference 500 amp dyno ad-1 full test wattage rms - the 1993the 1993soundstream reference500 tested on the smd / d'amore engineering amp dyno (ad-1). VIDEO 02 : memperbaiki power amplifier mobil soundstream reference 405s rusak satu chanel mati - power amplifier mobilpower amplifier mobilsoundstream reference 405srusaknya terlihat parah tapi ternyata penyebabnya sepele. VIDEO 01 : soundstream reference 405 amp guts-old school amp - i removed my old schooli removed my old schoolsound stream reference 405amp from my truck and wanted to let you take a look inside. ![]() To view the uncensored versions of these illustrations visit the MCL Illustration Viewer by Sakurina page. A list of these illustrations can be found on the Missing Wikia Illustrations The final string of numbers is the name of the illustration, if you know the code for another illustration, you can replace it to generate a new illustration.ĭue to Fandom's Terms of Use, some My Candy Love illustrations cannot be uploaded to the My Candy Love Wiki. Green refers to the eye colour this can be changed to another available MCL eye colour. Brown refers to the hair colour this can be changed to another available MCL hair colour. 1 specifies the skin colour this can be changed from 1 through 5, with 1 being the lightest skin tone. This is an example of the type of illustration url used in University Life, Love Life, and Alternate Life. University Life, Love Life and Alternate Life The final string of numbers is the name of the illustration, if you know the code for another illustration, you can replace it to generate a new illustration. ![]() ![]() Long specifies the hair length this can be changed to read short or curly. This is an example of a High School Life illustration url. If you know the url to a specific illustration, you can use it to modify the Candy featured to match yours. 1.2 University Life, Love Life and Alternate Life. ![]() Multani mitti is a great exfoliant that will help remove all the impurities on your face. ![]() Just mix it with your favourite ingredients and you are all set. Using multani mitti as a homemade face pack for glowing skin is the easiest way to use it. Here are some common ways of using multani mitti to attain a glowing complexion. It helps cleanse your skin and gets rid of impurities in your pores. This clay mask is a boon for those with acne-prone skin. Multani mitti detoxifies your skin and combats breakouts. ![]() It fades blemishes to unveil glowing and radiant skin. Multani mitti stimulates blood circulation and makes your skin supple. Multani mitti is ideal for oily skin types. It tightens your pores and removes excess sebum from the face. Multani mitti absorbs all the dirt and impurities from your pores, leaving it clean. This magical beauty ingredient can combat all forms of inflammation. This makes it the perfect remedy for sun burns. Multani mitti is a soothing ingredient that can help calm your skin. Regular usage can help attain a healthy and glowing complexion. If you don’t know how to get an even skin tone then this ingredient blessed with anti-tanning properties can give you an even-toned and brighter skin. Hence, works as a home remedy for whiteheads and blackheads. Multani mitti removes all the dead skin cells from your skin. Multani mitti can help remove all dirt, grime, and other impurities that could be present on your face. Here are 7 amazing benefits of using multani mitti. Using multani mitti for face has a number of benefits. Multani mitti is being used as a beauty ingredient since aeons. It’s a clay endowed with the goodness of minerals. Multani mitti is also known as Fuller’s earth. What is Multani Mitti For Skin (Fuller’s Earth)? From benefits of multani mitti for skin to how to use it, here’s everything you need to know about multani mitti. Multani mitti has a plethora of benefits that can make your skin healthy. Including this in your skincare routine will nourish and hydrate your skin. ![]() Pinta is also mentioned in our list of the 5 best Lightweight image editor for Linux. You can install Pinta in Linux as well as Mac OS and Windows OS also. This saves time when you just want to do simple and quick edits.Īs Pinta is open-source it’s available for free. NET 6 A huge thanks to the following contributors who worked on this release: cameronwhite, jpobst, darkdragon-001, thekolian1996, and iangzh. So that you could do simple edits fast and with ease. This is a major new release of Pinta, which has now been ported to GTK 3 and. It offers the simplicity of Paint while having some more advanced features also. It offers a very polished and clean user interface. But as moem said there are plenty to try. I doubt theres a close Linux equivalent to, and there definitely isnt one for Photoshop. Click on it and click the Install button. At the top right input box type in: Pinta and press Enter. ![]() Many Linux newbies seem to expect that theyll be able to run all their old Windows programs using wine, and that is a big mistake. You can install it using: Click on Ubuntu Software Center from Launcher (left site icon panel). Download pinta packages for Alpine, ALT Linux, Arch Linux, Fedora, FreeBSD, Mageia, OpenMandriva, openSUSE, PCLinuxOS, Red Hat Enterprise Linux, Solus. ![]() That’s where Pinta comes in, it’s almost similar to Paint.NET. Wine actually fails on most apps, its really a silly hack. For quick and fast learning and editing, we need an alternative to Paint for Linux. That is overkill for some easy and fast editing as well as have a steep learning curve. But unfortunately, it is not available for Linux systems.Īlthough there are many other editing software for Linux like GIMP, but it is more like photoshop. How to Install Pinta on Ubuntu 20.04 22.04 LTS Update your Ubuntu system packages with running the below command: sudo apt update -y & sudo. It’s small in size, light on hardware resources and is easy to learn. Once it is installed you can now use Pinta.If you have ever used Paint.NET on Windows operating system, then you should know how easy Paint.NET makes to edit images and graphics. Installing via the Ubuntu Software Center Run this command: sudo apt-get install pinta Ubuntu 12.04 sudo add-apt-repository ppa:pinta-maintainers/pinta-stable Ubuntu 10.10, 11.04 & 11.10 sudo add-apt-repository ppa:pinta-maintainers/pinta-stable/ubuntu Installationįor Ubuntu versions up to 12.04 you need to add a PPA to install this and keep it updated: It preserves every edit you have made so that you can reverse the changes. Say you want to continue a work later on, keeping all the layers intact (so that you can add/remove them later on), you can save the file in.
During that time, and until the 1800s, almost all speaking was argumentative. Ancient philosophers and statesmen like Aristotle, Cicero, and Quintilian conceived of public speaking as rhetoric, which is inherently persuasive. Interestingly, informative speaking is a newcomer in the world of public speaking theorizing and instruction, which began thousands of years ago with the ancient Greeks (Olbricht, 1968). The goal of informative speaking is to teach an audience something using objective factual information. Since your exposure to informative speaking is inevitable, why not learn how to be a better producer and consumer of informative messages?Īs you’ll recall from the chapter titled “Preparing a Speech”, speaking to inform is one of the three possible general purposes for public speaking. College alumni have reported that out of many different speech skills, informative speaking is most important (Verderber, 1991). Some organizations, like consulting firms, and career fields, like training and development, are solely aimed at conveying information. That means you are more likely to give and listen to informative speeches in a variety of contexts. Reports, lectures, training seminars, and demonstrations are all examples of informative speaking. Although informative speaking may not be the most exciting form of public speaking, it is the most common. Many people would rather go see an impassioned political speech or a comedic monologue than a lecture. ![]() Employ strategies for effective informative speaking, including avoiding persuasion, avoiding information overload, and engaging the audience.Explain the different methods of informing.Identify strategies for researching and supporting informative speeches.Identify common topic categories for informative speeches. ![]() Some people may worry that their counselor is not accredited. What are some common concerns about online therapy? By looking at the diverse experiences of others, you can see how it might benefit you. To understand how other users have felt about a platform like BetterHelp, it may be helpful to look at past reviews. Session options may include video and phone calls or live chat with your counselor, which allows you control over how you receive support. In addition to effectiveness, online counseling offers the convenience of allowing clients to message their therapist throughout the week and outside of sessions to receive responses when they are available. Research shows that online therapy is as effective as in-person psychotherapy when working with a licensed clinical therapist. The effectiveness of therapy has been proven through hundreds of studies and clinical trials. Although some people may worry about reaching out for help due to stigma, online therapy is for everyone, regardless of age, gender, sexuality, race, ethnicity, or background. For example, therapists can support clients with depression, anxiety, chronic stress, relationship conflicts, career challenges, bipolar disorder, personality disorders, traumatic events, and other topics. A counselor is trained to offer guidance in many areas and may specialize in several modalities and symptoms. You don’t have to have a mental illness or diagnosis to seek support. How can online therapy help me?Īnyone can use online counseling. These options might include group psychotherapy sessions, journaling prompts, webinar classes, and thousands of articles on mental health topics. Platforms like BetterHelp may also offer extra features, which may not be offered by in-person therapists, increasing the cost-benefit. This pricing is more cost-effective than the national US market average of $125 to $199 per session. ![]() BetterHelp’s subscriptions range from $60 to $90 per week (billed every four weeks) for unlimited messaging and a weekly video, phone, or live chat session with a licensed mental health professional. Another study, published in JAMA Network Open, reports that online therapy can be more cost-effective, especially in treating conditions like obsessive-compulsive disorder (OCD).įrequently asked questions How much is online therapy? The researchers also conclude that virtual or internet therapy can increase reported quality of life, satisfaction with treatment, and recovery rates. One study, accessed in the National Library of Medicine, showcases how cognitive-behavioral therapy can effectively treat major depressive disorder and anxiety. Telehealth therapy has been proven effective by multiple studies. For those who can’t commute to an appointment due to remote location, income insecurity, or a disability, online therapy can allow accessibility that may not be found elsewhere. ![]() In addition, it allows clients to take advantage of affordable, thorough mental health services without using health insurance. Sessions can be accessed from home or anywhere with a wifi connection. Since 2021, four out of ten Americans have used online counseling services to meet with their therapist, often due to the convenience, accessibility, and affordability of this treatment option. Online therapy has guided millions of clients worldwide. Interactive visualizations and dashboards with modern designĪdvanced visualization capabilities, complex charts and mapsĮasy sharing within Power BI service, integration with SharePoint and Teams Native connectors to Microsoft products and various data sourcesīroad compatibility with databases, web services, and file formats Steeper learning curve and requires more technical skills User-friendly with a drag-and-drop interface List out differences between Power BI and Tableau Aspectĭeveloped by Tableau Software (now part of Salesforce) Power BI Basic Interview Questions – General Questions 1. For your ease of access, I have categorized the Power BI interview questions namely: So, here are the Top 50 Power BI Interview Questions and Answers which are most likely to be asked by the interviewer. You can even get Power BI Training in Delhi or in any nearby city. If you want to brush up with the Power BI basics, you can take a look at this Power BI Tutorial blog. These questions are collected after consulting with top industry experts in the field of Data analytics and visualization. In this Power BI interview questions and answers blog, I have collected the most frequently asked questions by interviewers. ![]() ![]() In case you are still not convinced about moving into the Power BI domain, the below image will give you an idea about the current market for Power BI: ![]() Below image is the change in positions of organizations in the Data Visualization domain as per Gartner:Īs you can see there is a major moment of Microsoft in the year 2017 when compared to 2016 and this is mainly due to the contribution oferal Power BI. Power BI has been the major contributing factor for Microsoft’s growth in the domain of Business Intelligence and Data Visualization in the year 2016-17. Power BI came into existence in late 2013 after Microsoft had decided to combine multiples excel add-ons to create a completely new and independent tool. In this Power BI Interview Questions blog, we will be discussing some of the most important interview questions associated with Power BI which will help you stand out in your interview. |
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